RapamycinFungusV1, Main, Exploration, bibRecord, 000475

TSC1 and DEPDC5 regulate HIV-1 latency through the mTOR signaling pathway.

Identifieur interne : 000475 ( Main/Exploration ); précédent : 000474; suivant : 000476

TSC1 and DEPDC5 regulate HIV-1 latency through the mTOR signaling pathway.

Auteurs : Shan Jin [République populaire de Chine] ; Qibin Liao [République populaire de Chine] ; Jian Chen [République populaire de Chine] ; Linxia Zhang [République populaire de Chine] ; Qian He [République populaire de Chine] ; Huanzhang Zhu [République populaire de Chine] ; Xiaoyan Zhang [République populaire de Chine] ; Jianqing Xu [République populaire de Chine]

Source :

Emerging microbes & infections [ 2222-1751 ] ; 2018.

RBID : pubmed:30087333

Descripteurs français

English descriptors

Abstract

The latent reservoir of HIV-1 presents a major barrier to viral eradication. The mechanism of the establishment and maintenance of the latent viral reservoir is not yet fully understood, which hinders the development of effective curative strategies. In this study, we identified two inhibitory genes, TSC1 and DEPDC5, that maintained HIV-1 latency by suppressing the mTORC1 pathway. We first adapted a genome-wide CRISPR screening approach to identify host factors required for HIV latency in a T-cell-based latency model and discovered two inhibitory genes, TSC1 and DEPDC5, which are potentially involved in HIV-1 latency. Knockout of either TSC1 or DEPDC5 led to enhanced HIV-1 reactivation in both a T-cell line (C11) and a monocyte cell line (U1), and this enhancement could be antagonized by the mTORC1 inhibitor rapamycin. Further evaluation of the mechanism revealed that TSC1 suppresses AKT-mTORC1-S6 via downregulation of Rheb, whereas DEPDC5 inhibits AKT-mTORC1-S6 through RagA. Overall, both TSC1 and DEPDC5 negatively regulate the AKT-mTORC1 pathway, and thus their agonists could be used in the development of new therapeutic approaches for activating HIV-1 latency.

DOI: 10.1038/s41426-018-0139-5
PubMed: 30087333
PubMed Central: PMC6081400

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000484
to stream Main, to step Curation: 000484

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">TSC1 and DEPDC5 regulate HIV-1 latency through the mTOR signaling pathway.</title>
<author><name sortKey="Jin, Shan" sort="Jin, Shan" uniqKey="Jin S" first="Shan" last="Jin">Shan Jin</name>
<affiliation wicri:level="1"><nlm:affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Liao, Qibin" sort="Liao, Qibin" uniqKey="Liao Q" first="Qibin" last="Liao">Qibin Liao</name>
<affiliation wicri:level="1"><nlm:affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chen, Jian" sort="Chen, Jian" uniqKey="Chen J" first="Jian" last="Chen">Jian Chen</name>
<affiliation wicri:level="1"><nlm:affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Linxia" sort="Zhang, Linxia" uniqKey="Zhang L" first="Linxia" last="Zhang">Linxia Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="He, Qian" sort="He, Qian" uniqKey="He Q" first="Qian" last="He">Qian He</name>
<affiliation wicri:level="1"><nlm:affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhu, Huanzhang" sort="Zhu, Huanzhang" uniqKey="Zhu H" first="Huanzhang" last="Zhu">Huanzhang Zhu</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Genetic Engineering and Key Laboratory of Medical Molecular Virology of Ministry of Education/Health, School of Life Sciences, Fudan University, Shanghai, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Genetic Engineering and Key Laboratory of Medical Molecular Virology of Ministry of Education/Health, School of Life Sciences, Fudan University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Xiaoyan" sort="Zhang, Xiaoyan" uniqKey="Zhang X" first="Xiaoyan" last="Zhang">Xiaoyan Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China. zhangxiaoyan@shphc.org.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xu, Jianqing" sort="Xu, Jianqing" uniqKey="Xu J" first="Jianqing" last="Xu">Jianqing Xu</name>
<affiliation wicri:level="1"><nlm:affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China. xujianqing@shphc.org.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
</affiliation>
<affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory for Infectious Disease Prevention and Control, China Centers for Disease Control and Prevention, Beijing, China. xujianqing@shphc.org.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory for Infectious Disease Prevention and Control, China Centers for Disease Control and Prevention, Beijing</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2018">2018</date>
<idno type="RBID">pubmed:30087333</idno>
<idno type="pmid">30087333</idno>
<idno type="doi">10.1038/s41426-018-0139-5</idno>
<idno type="pmc">PMC6081400</idno>
<idno type="wicri:Area/Main/Corpus">000484</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000484</idno>
<idno type="wicri:Area/Main/Curation">000484</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000484</idno>
<idno type="wicri:Area/Main/Exploration">000484</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">TSC1 and DEPDC5 regulate HIV-1 latency through the mTOR signaling pathway.</title>
<author><name sortKey="Jin, Shan" sort="Jin, Shan" uniqKey="Jin S" first="Shan" last="Jin">Shan Jin</name>
<affiliation wicri:level="1"><nlm:affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Liao, Qibin" sort="Liao, Qibin" uniqKey="Liao Q" first="Qibin" last="Liao">Qibin Liao</name>
<affiliation wicri:level="1"><nlm:affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chen, Jian" sort="Chen, Jian" uniqKey="Chen J" first="Jian" last="Chen">Jian Chen</name>
<affiliation wicri:level="1"><nlm:affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Linxia" sort="Zhang, Linxia" uniqKey="Zhang L" first="Linxia" last="Zhang">Linxia Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="He, Qian" sort="He, Qian" uniqKey="He Q" first="Qian" last="He">Qian He</name>
<affiliation wicri:level="1"><nlm:affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhu, Huanzhang" sort="Zhu, Huanzhang" uniqKey="Zhu H" first="Huanzhang" last="Zhu">Huanzhang Zhu</name>
<affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Genetic Engineering and Key Laboratory of Medical Molecular Virology of Ministry of Education/Health, School of Life Sciences, Fudan University, Shanghai, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory of Genetic Engineering and Key Laboratory of Medical Molecular Virology of Ministry of Education/Health, School of Life Sciences, Fudan University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Xiaoyan" sort="Zhang, Xiaoyan" uniqKey="Zhang X" first="Xiaoyan" last="Zhang">Xiaoyan Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China. zhangxiaoyan@shphc.org.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xu, Jianqing" sort="Xu, Jianqing" uniqKey="Xu J" first="Jianqing" last="Xu">Jianqing Xu</name>
<affiliation wicri:level="1"><nlm:affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China. xujianqing@shphc.org.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai</wicri:regionArea>
<wicri:noRegion>Shanghai</wicri:noRegion>
</affiliation>
<affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory for Infectious Disease Prevention and Control, China Centers for Disease Control and Prevention, Beijing, China. xujianqing@shphc.org.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>State Key Laboratory for Infectious Disease Prevention and Control, China Centers for Disease Control and Prevention, Beijing</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Emerging microbes & infections</title>
<idno type="eISSN">2222-1751</idno>
<imprint><date when="2018" type="published">2018</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Line (MeSH)</term>
<term>HIV Infections (enzymology)</term>
<term>HIV Infections (genetics)</term>
<term>HIV Infections (metabolism)</term>
<term>HIV Infections (virology)</term>
<term>HIV-1 (genetics)</term>
<term>HIV-1 (physiology)</term>
<term>Host-Pathogen Interactions (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Mechanistic Target of Rapamycin Complex 1 (genetics)</term>
<term>Mechanistic Target of Rapamycin Complex 1 (metabolism)</term>
<term>Monomeric GTP-Binding Proteins (genetics)</term>
<term>Monomeric GTP-Binding Proteins (metabolism)</term>
<term>Proto-Oncogene Proteins c-akt (genetics)</term>
<term>Proto-Oncogene Proteins c-akt (metabolism)</term>
<term>Ras Homolog Enriched in Brain Protein (genetics)</term>
<term>Ras Homolog Enriched in Brain Protein (metabolism)</term>
<term>Repressor Proteins (genetics)</term>
<term>Repressor Proteins (metabolism)</term>
<term>Signal Transduction (MeSH)</term>
<term>T-Lymphocytes (metabolism)</term>
<term>T-Lymphocytes (virology)</term>
<term>Tuberous Sclerosis Complex 1 Protein (MeSH)</term>
<term>Tumor Suppressor Proteins (genetics)</term>
<term>Tumor Suppressor Proteins (metabolism)</term>
<term>Virus Activation (MeSH)</term>
<term>Virus Latency (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Activation virale (MeSH)</term>
<term>Complexe-1 cible mécanistique de la rapamycine (génétique)</term>
<term>Complexe-1 cible mécanistique de la rapamycine (métabolisme)</term>
<term>Humains (MeSH)</term>
<term>Infections à VIH (enzymologie)</term>
<term>Infections à VIH (génétique)</term>
<term>Infections à VIH (métabolisme)</term>
<term>Infections à VIH (virologie)</term>
<term>Interactions hôte-pathogène (MeSH)</term>
<term>Latence virale (MeSH)</term>
<term>Lignée cellulaire (MeSH)</term>
<term>Lymphocytes T (métabolisme)</term>
<term>Lymphocytes T (virologie)</term>
<term>Protéine homologue de Ras enrichie dans le cerveau (génétique)</term>
<term>Protéine homologue de Ras enrichie dans le cerveau (métabolisme)</term>
<term>Protéine-1 du complexe de la sclérose tubéreuse (MeSH)</term>
<term>Protéines G monomériques (génétique)</term>
<term>Protéines G monomériques (métabolisme)</term>
<term>Protéines de répression (génétique)</term>
<term>Protéines de répression (métabolisme)</term>
<term>Protéines proto-oncogènes c-akt (génétique)</term>
<term>Protéines proto-oncogènes c-akt (métabolisme)</term>
<term>Protéines suppresseurs de tumeurs (génétique)</term>
<term>Protéines suppresseurs de tumeurs (métabolisme)</term>
<term>Transduction du signal (MeSH)</term>
<term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique)</term>
<term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Mechanistic Target of Rapamycin Complex 1</term>
<term>Monomeric GTP-Binding Proteins</term>
<term>Proto-Oncogene Proteins c-akt</term>
<term>Ras Homolog Enriched in Brain Protein</term>
<term>Repressor Proteins</term>
<term>Tumor Suppressor Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Infections à VIH</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>HIV Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV Infections</term>
<term>HIV-1</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Complexe-1 cible mécanistique de la rapamycine</term>
<term>Infections à VIH</term>
<term>Protéine homologue de Ras enrichie dans le cerveau</term>
<term>Protéines G monomériques</term>
<term>Protéines de répression</term>
<term>Protéines proto-oncogènes c-akt</term>
<term>Protéines suppresseurs de tumeurs</term>
<term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>HIV Infections</term>
<term>Mechanistic Target of Rapamycin Complex 1</term>
<term>Monomeric GTP-Binding Proteins</term>
<term>Proto-Oncogene Proteins c-akt</term>
<term>Ras Homolog Enriched in Brain Protein</term>
<term>Repressor Proteins</term>
<term>T-Lymphocytes</term>
<term>Tumor Suppressor Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Complexe-1 cible mécanistique de la rapamycine</term>
<term>Infections à VIH</term>
<term>Lymphocytes T</term>
<term>Protéine homologue de Ras enrichie dans le cerveau</term>
<term>Protéines G monomériques</term>
<term>Protéines de répression</term>
<term>Protéines proto-oncogènes c-akt</term>
<term>Protéines suppresseurs de tumeurs</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>HIV-1</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à VIH</term>
<term>Lymphocytes T</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>HIV Infections</term>
<term>T-Lymphocytes</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Cell Line</term>
<term>Host-Pathogen Interactions</term>
<term>Humans</term>
<term>Signal Transduction</term>
<term>Tuberous Sclerosis Complex 1 Protein</term>
<term>Virus Activation</term>
<term>Virus Latency</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Activation virale</term>
<term>Humains</term>
<term>Interactions hôte-pathogène</term>
<term>Latence virale</term>
<term>Lignée cellulaire</term>
<term>Protéine-1 du complexe de la sclérose tubéreuse</term>
<term>Transduction du signal</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The latent reservoir of HIV-1 presents a major barrier to viral eradication. The mechanism of the establishment and maintenance of the latent viral reservoir is not yet fully understood, which hinders the development of effective curative strategies. In this study, we identified two inhibitory genes, TSC1 and DEPDC5, that maintained HIV-1 latency by suppressing the mTORC1 pathway. We first adapted a genome-wide CRISPR screening approach to identify host factors required for HIV latency in a T-cell-based latency model and discovered two inhibitory genes, TSC1 and DEPDC5, which are potentially involved in HIV-1 latency. Knockout of either TSC1 or DEPDC5 led to enhanced HIV-1 reactivation in both a T-cell line (C11) and a monocyte cell line (U1), and this enhancement could be antagonized by the mTORC1 inhibitor rapamycin. Further evaluation of the mechanism revealed that TSC1 suppresses AKT-mTORC1-S6 via downregulation of Rheb, whereas DEPDC5 inhibits AKT-mTORC1-S6 through RagA. Overall, both TSC1 and DEPDC5 negatively regulate the AKT-mTORC1 pathway, and thus their agonists could be used in the development of new therapeutic approaches for activating HIV-1 latency.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30087333</PMID>
<DateCompleted><Year>2018</Year>
<Month>11</Month>
<Day>02</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>12</Month>
<Day>02</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2222-1751</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>7</Volume>
<Issue>1</Issue>
<PubDate><Year>2018</Year>
<Month>Aug</Month>
<Day>08</Day>
</PubDate>
</JournalIssue>
<Title>Emerging microbes & infections</Title>
<ISOAbbreviation>Emerg Microbes Infect</ISOAbbreviation>
</Journal>
<ArticleTitle>TSC1 and DEPDC5 regulate HIV-1 latency through the mTOR signaling pathway.</ArticleTitle>
<Pagination><MedlinePgn>138</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1038/s41426-018-0139-5</ELocationID>
<Abstract><AbstractText>The latent reservoir of HIV-1 presents a major barrier to viral eradication. The mechanism of the establishment and maintenance of the latent viral reservoir is not yet fully understood, which hinders the development of effective curative strategies. In this study, we identified two inhibitory genes, TSC1 and DEPDC5, that maintained HIV-1 latency by suppressing the mTORC1 pathway. We first adapted a genome-wide CRISPR screening approach to identify host factors required for HIV latency in a T-cell-based latency model and discovered two inhibitory genes, TSC1 and DEPDC5, which are potentially involved in HIV-1 latency. Knockout of either TSC1 or DEPDC5 led to enhanced HIV-1 reactivation in both a T-cell line (C11) and a monocyte cell line (U1), and this enhancement could be antagonized by the mTORC1 inhibitor rapamycin. Further evaluation of the mechanism revealed that TSC1 suppresses AKT-mTORC1-S6 via downregulation of Rheb, whereas DEPDC5 inhibits AKT-mTORC1-S6 through RagA. Overall, both TSC1 and DEPDC5 negatively regulate the AKT-mTORC1 pathway, and thus their agonists could be used in the development of new therapeutic approaches for activating HIV-1 latency.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jin</LastName>
<ForeName>Shan</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Liao</LastName>
<ForeName>Qibin</ForeName>
<Initials>Q</Initials>
<AffiliationInfo><Affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Jian</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Linxia</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>He</LastName>
<ForeName>Qian</ForeName>
<Initials>Q</Initials>
<AffiliationInfo><Affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhu</LastName>
<ForeName>Huanzhang</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Genetic Engineering and Key Laboratory of Medical Molecular Virology of Ministry of Education/Health, School of Life Sciences, Fudan University, Shanghai, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Xiaoyan</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China. zhangxiaoyan@shphc.org.cn.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Xu</LastName>
<ForeName>Jianqing</ForeName>
<Initials>J</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0003-0896-9273</Identifier>
<AffiliationInfo><Affiliation>Shanghai Public Health Clinical Center and Institutes of Biomedical Sciences, Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, Shanghai, China. xujianqing@shphc.org.cn.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>State Key Laboratory for Infectious Disease Prevention and Control, China Centers for Disease Control and Prevention, Beijing, China. xujianqing@shphc.org.cn.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>81561128008</GrantID>
<Agency>National Natural Science Foundation of China (National Science Foundation of China)</Agency>
<Country></Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2018</Year>
<Month>08</Month>
<Day>08</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Emerg Microbes Infect</MedlineTA>
<NlmUniqueID>101594885</NlmUniqueID>
<ISSNLinking>2222-1751</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C000590398">DEPDC5 protein, human</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000076205">Ras Homolog Enriched in Brain Protein</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012097">Repressor Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C000624650">TSC1 protein, human</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000077004">Tuberous Sclerosis Complex 1 Protein</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D025521">Tumor Suppressor Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 2.7.11.1</RegistryNumber>
<NameOfSubstance UI="D000076222">Mechanistic Target of Rapamycin Complex 1</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 2.7.11.1</RegistryNumber>
<NameOfSubstance UI="D051057">Proto-Oncogene Proteins c-akt</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.6.1.-</RegistryNumber>
<NameOfSubstance UI="C484342">RRAGA protein, human</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 3.6.5.2</RegistryNumber>
<NameOfSubstance UI="D020559">Monomeric GTP-Binding Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015658" MajorTopicYN="N">HIV Infections</DescriptorName>
<QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015497" MajorTopicYN="N">HIV-1</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000076222" MajorTopicYN="N">Mechanistic Target of Rapamycin Complex 1</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020559" MajorTopicYN="N">Monomeric GTP-Binding Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051057" MajorTopicYN="N">Proto-Oncogene Proteins c-akt</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000076205" MajorTopicYN="N">Ras Homolog Enriched in Brain Protein</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012097" MajorTopicYN="N">Repressor Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013601" MajorTopicYN="N">T-Lymphocytes</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000077004" MajorTopicYN="N">Tuberous Sclerosis Complex 1 Protein</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D025521" MajorTopicYN="N">Tumor Suppressor Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014775" MajorTopicYN="N">Virus Activation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017735" MajorTopicYN="Y">Virus Latency</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year>
<Month>04</Month>
<Day>15</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2018</Year>
<Month>06</Month>
<Day>23</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2018</Year>
<Month>06</Month>
<Day>14</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2018</Year>
<Month>8</Month>
<Day>9</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2018</Year>
<Month>8</Month>
<Day>9</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2018</Year>
<Month>11</Month>
<Day>6</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">30087333</ArticleId>
<ArticleId IdType="doi">10.1038/s41426-018-0139-5</ArticleId>
<ArticleId IdType="pii">10.1038/s41426-018-0139-5</ArticleId>
<ArticleId IdType="pmc">PMC6081400</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>J Virol. 2011 Jun;85(12):6060-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21471244</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Leukoc Biol. 2012 Dec;92(6):1147-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22802445</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Development. 2012 May;139(9):1557-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22492351</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Am J Transplant. 2014 May;14(5):1136-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24698537</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1999 Sep 24;274(39):27981-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10488148</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Cancer. 2003 Nov 09;3:30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14606959</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2010 Mar 16;107(11):5012-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20194747</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2016 Dec 16;91(1):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27795436</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbiol Immunol. 2011 Jan;55(1):1-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21175768</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Neurovirol. 2012 Oct;18(5):400-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22618514</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2005 Jun;79(11):6610-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15890899</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Host Microbe. 2011 Nov 17;10(5):426-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22100159</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS Lett. 2017 Oct;591(20):3319-3332</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28892135</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2001 Jul 27;276(30):28179-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11384967</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Med Rep. 2018 Feb;17(2):2501-2508</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29207194</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 1993 Sep;12(9):3551-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8253080</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2014 Nov 13;10(10):e1004473</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25393648</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2016 Jul 22;353(6297):aaf6517</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27463679</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 2014 Apr;20(4):425-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24658076</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2011 Jun;85(11):5384-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21430059</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 2007 Oct;13(10):1241-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17906637</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Med. 2015 Nov 20;13:284</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26588898</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Viruses. 2014 Apr 14;6(4):1715-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24736215</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Blood. 2010 Jun 10;115(23):4742-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20351312</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2012 Jul 25;487(7408):482-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22837004</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>F1000Res. 2018 Jan 30;7:125</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29445452</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Retrovirology. 2016 Dec 20;13(1):86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27998285</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Immunol. 2012;30:39-68</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22136167</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 1996 Mar 1;15(5):1112-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8605881</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2013 May 31;340(6136):1100-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23723238</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2007 Jan 24;26(2):412-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17245431</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2015 Jun 11;11(6):e1004955</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26067822</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 2016 Jun 1;196(11):4437-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27207806</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Retrovirology. 2013 Aug 16;10:90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23953889</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Antimicrob Agents Chemother. 2002 Nov;46(11):3447-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12384349</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Mol Cell Biol. 2014 Mar;15(3):155-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24556838</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Microbiol. 2016 Feb;24(2):90-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26690612</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Host Microbe. 2016 Dec 14;20(6):785-797</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27978436</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunity. 2017 Oct 17;47(4):766-775.e3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29045905</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2015 Jan;89(1):615-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25339774</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2012;8(7):e1002818</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22911005</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Expert Opin Ther Targets. 2017 Jun;21(6):591-600</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28406046</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2013 Oct 30;8(10):e77749</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24204950</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 1995 Dec;1(12):1284-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7489410</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2007 Dec 12;26(24):4985-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18007589</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2011 Apr;85(7):3187-202</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21270164</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1987 Apr 16-22;326(6114):711-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3031512</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Methods. 2014 Aug;11(8):783-784</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25075903</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>AIDS. 2015 Jan 28;29(3):275-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25490467</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2015 Jul 28;112(30):9412-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26170311</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2013 Oct 24;155(3):519-29</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24243012</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2014 Apr;454-455:328-39</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24565118</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Microbiol. 2014 Nov;12(11):750-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25402363</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 2011 Mar 15;186(6):3299-303</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21317389</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Invest. 2017 Feb 1;127(2):651-656</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28094770</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Mol Cell Biol. 2013 Mar;14(3):133-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23361334</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genes Dev. 2003 Aug 1;17(15):1829-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12869586</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cold Spring Harb Perspect Med. 2011 Sep;1(1):a007096</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22229121</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Immunol. 2004 Jun;34(6):1705-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15162441</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Host Microbe. 2008 Aug 14;4(2):123-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18692772</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Exp Hematol. 2017 Jun;50:13-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28342808</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):2927-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9096323</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Retrovirology. 2013 Jun 26;10:67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23803414</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1987 Nov 6;238(4828):800-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3313729</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Rep. 2017 Aug 15;20(7):1503-1512</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28813663</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunol Rev. 2013 Jul;254(1):305-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23772628</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2008 Dec;82(24):12291-303</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18829756</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2007 Jan 24;26(2):424-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17245432</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2012 Sep;86(17):9495-503</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22674979</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<settlement><li>Pékin</li>
</settlement>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Jin, Shan" sort="Jin, Shan" uniqKey="Jin S" first="Shan" last="Jin">Shan Jin</name>
</noRegion>
<name sortKey="Chen, Jian" sort="Chen, Jian" uniqKey="Chen J" first="Jian" last="Chen">Jian Chen</name>
<name sortKey="He, Qian" sort="He, Qian" uniqKey="He Q" first="Qian" last="He">Qian He</name>
<name sortKey="Liao, Qibin" sort="Liao, Qibin" uniqKey="Liao Q" first="Qibin" last="Liao">Qibin Liao</name>
<name sortKey="Xu, Jianqing" sort="Xu, Jianqing" uniqKey="Xu J" first="Jianqing" last="Xu">Jianqing Xu</name>
<name sortKey="Xu, Jianqing" sort="Xu, Jianqing" uniqKey="Xu J" first="Jianqing" last="Xu">Jianqing Xu</name>
<name sortKey="Zhang, Linxia" sort="Zhang, Linxia" uniqKey="Zhang L" first="Linxia" last="Zhang">Linxia Zhang</name>
<name sortKey="Zhang, Xiaoyan" sort="Zhang, Xiaoyan" uniqKey="Zhang X" first="Xiaoyan" last="Zhang">Xiaoyan Zhang</name>
<name sortKey="Zhu, Huanzhang" sort="Zhu, Huanzhang" uniqKey="Zhu H" first="Huanzhang" last="Zhu">Huanzhang Zhu</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RapamycinFungusV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000475 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000475 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    RapamycinFungusV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:30087333
   |texte=   TSC1 and DEPDC5 regulate HIV-1 latency through the mTOR signaling pathway.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:30087333" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a RapamycinFungusV1

This area was generated with Dilib version V0.6.38.
Data generation: Thu Nov 19 21:55:41 2020. Site generation: Thu Nov 19 22:00:39 2020

	Serveur d'exploration sur la rapamycine et les champignons
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur la rapamycine et les champignons

TSC1 and DEPDC5 regulate HIV-1 latency through the mTOR signaling pathway.

TSC1 and DEPDC5 regulate HIV-1 latency through the mTOR signaling pathway.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki